| Formulation | H2O |
| MolecularWeight | |
| Storage | -80°C |
| Purity | HPLCandTLCanalysis |
| Compound | |
| Assay | N/A |
| ShelfLife(properlystored) | 12months |
| ChemicalFormula | |
ThestructureofdansylarginineN-(3-ethyl-1,5-pentanediyl)amide(DAPA)isillustrated.DAPAisapotentinhibitorofthrombinwhichexhibitsuniquechangesinitsfluorescentpropertieswhenboundtothrombin.
Overview:
DansylarginineN-(3-ethyl-1,5-pentanediyl)amide,morecommonlyreferredtoasDAPA,isapotent(Ki=10-7M)andspecificsyntheticthrombininhibitor(1).OfspecialinterestaretheuniquefluorescentpropertiescontributedbythedansylmoietyofDAPA.Whenboundtothrombin,thefluorescenceintensityandlifetimeofthedansylmoietyareincreasedthreefold.Theenhancementoffluorescenceintensity,coupledwithitsinhibitoryproperties,havemadethiscompoundextremelyusefulforstudiesinvolvingthrombingeneration(2-7).Inadditiontoanincreaseinfluorescenceintensityandlifetime,thereisadecreaseindepolarizationoftheexcitationsignalwhichalsosupportsfluorescencepolarizationstudies.
TheinhibitorpropertiesofDAPAalonehavebeenusefulinavarietyofapplications.DAPAisroutinelyusedasaproteaseinhibitorduringtheisolationofproteinsthataresusceptIBLetothrombincleavage.FurThermore,byeliminatingthefeedbackofthrombinactivityduringprothrombinactivation,theisolationofreactionintermediatessuchasfragment1.2,andmeizothrombinhavebeenmadepossible(7).
DAPAissynthesizedaccordingtheproceduredescribedbyNesheim,etal.(1).HPLC,TLCandspectralanalysesareusedtodeterminethepurityofthefinalproduct.DAPAissuppliedinwater.Whenproperlystoredat-20°Candprotectedfromlight,thecompoundisstableformanyyears.
Properties:
| Formula | C25H39O3N6S1Cl1(reference#1) DansylarginineN-(3-ethyl-1,5-pentanediyl)amide | |||||
|---|---|---|---|---|---|---|
| Molecularweight | 539g/mole(1) | |||||
| Extinctioncoefficient |
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| Specialproperties | Aspecificandpotentthrombininhibitor(Ki=10-7M).Exhibitsa3-foldincreaseinfluorescenceintensityandlifetimewhenboundtothrombin. |
