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当前位置: 首页 > 产品中心 > globulin > Haematologic Technologies/Bovine Bone Osteocalcin/BOC-3020/100 µg
商品详细Haematologic Technologies/Bovine Bone Osteocalcin/BOC-3020/100 µg
Haematologic Technologies/Bovine Bone Osteocalcin/BOC-3020/100 µg
Haematologic Technologies/Bovine Bone Osteocalcin/BOC-3020/100 µg
商品编号: BOC-3020
品牌: haemtech
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 球蛋白
公司分类: globulin
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Formulation50%Glycerol/H2O(v/v)
Storage-20°C
Purity>95%bySDS-PAGE
ActivityDeterminationN/A
ShelfLife(properlystored)12months
AminoAcidSequenceofBovineOsteocalcin
Theaminoacidsequenceofbovineboneosteocalcin.Thepositionsoftheg-carboxyglutamicacidresiduesareindicatedbygamma.

SampleGelInformation:

GelNovex4-12%Bis-Tris
LoadBovineosteocalcin,1µgperlane
BufferMES
StandardSeeBluePlus2;Myosin(188kDa),PhosphorylaseB(98kDa),BSA(62kDa),GlutamicDehydrogenase(49kDa),AlcoholDehydrogenase(38kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(17kDa),Lysozyme(14kDa),Aprotinin(6kDa),Insulin,Bchain(3kDa).

Overview:

Osteocalcin,alsoknownasBone-Gla-Protein(BGP),isasinglechain(Mr=5800)vitaminKdependentproteinproducedbyosteoblastsandfoundinhighconcentrationsinbone(1-4).Post-translationalmodificationbyavitaminKdependentcarboxylaseproducesthreeg-carboxyglutamicacidresiduesatpositions17,21and24.Thematureproteincontains49aminoacidsandasingleintrachaindisulfidebondjoiningCys23toCys29.Thesecondarystructureishighlycalciumdependentandcontains14%α-helix,20%β-sheetand67%randomforminthepresenceofcalcium,and1%α-helix,20%β-sheetand79%randomformintheabsenceofcalcium(4).

BGPbindstophospholipidvesiclesinthepresenceofcalciumions(Kd=6x10-6M)(5).BGPalsobindshydroxyapatiteandisanefficientinhibitorofhydroxyapatiteseededcrystalgrowth,suggestingaregulatoryroleinbonemineralization(6).AlthoughthefundamentalroleofBGPinvivoremainselusive,invitroexperimentationsuggestsrolesinbothboneresorptionandboneformation.BGPhasbeenshowntofunctionasachemo-attractantinaninvitromacrophagemediatedboneresorptionsystem.TheserumconcentrationofBGPhasbeenlinkedtoboneformationrates,andBGPlevelsarecurrentlyusedinresearchassessmentofosteoporosisandothermetabolicbonediseasestates(7).

OsteocalcinispreparedfrombovinebonegenerallyasdescribedbyPriceetal.(1).ThisinvolvesdemineralizationinEDTA,sizeexclusionandionexchangechromatography.Thepurifiedproteinissupplied50%(vol/vol)glycerol/0.01MTris,0.075MNaCl,pH7.4,andshouldbestoredat-20°C.Humanosteocalcinispreparedfromextractsofacid-demineralizedbone,usingimmunoaffinitychromatography.Thehumanproteinissuppliedin0.02MTris,0.15MNaCl,2mMCaCl2,pH7.4andshouldbestoredat-80°C.PurityisdeterminedbySDS-PAGE.

Properties:

LocalizationBone,plasma
ModeofactionUnknown
Molecularweight5,800(1)
Extinctioncoefficient
E
1%
1cm,280nm
=13.3(7)(calculated)
Isoelectricpoint4.0-4.5(8)
StructureSinglechain,oneintrachaindisulfidebond,Cys23-29.
Percentcarbohydrate0%
Post-translationalmodificationsThreeglaresidues,17,21,24
品牌介绍
Haematologic Technologies是一家通过ISO 9001:2015认证的公司,是一家主要制造商,专门从事旨在用于体外研究的高质量血浆蛋白的分离和表征的主要制造商。HT的重点是参与凝血级联反应的蛋白质,以及骨代谢的调节。HT产品系列包括150多种高度纯化且特性良好的蛋白质,包括酶原,酶,辅因子和抑制剂,以及单克隆和多克隆抗体的互补产品系列。还提供因子不足的血浆和定制的用于临床研究的定制采血管。提供的服务包括定制蛋白质纯化,蛋白质修饰,分析开发,合同制造和合同研究。