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当前位置: 首页 > 产品中心 > Cell_lysis > 血液学技术/人肝素辅因子II-血液学技术公司/HCII-0190/HCII-0190-100µg
商品详细血液学技术/人肝素辅因子II-血液学技术公司/HCII-0190/HCII-0190-100µg
血液学技术/人肝素辅因子II-血液学技术公司/HCII-0190/HCII-0190-100µg
血液学技术/人肝素辅因子II-血液学技术公司/HCII-0190/HCII-0190-100µg
商品编号: HCII-0190-100µg
品牌: haemtech
市场价: ¥7900.00
美元价: 4740.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞裂解和提取物
公司分类: Cell_lysis
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Heparin cofactor II (HCII) (heparin cofactor A, antithrombin BM, dermatan sulfate cofactor, human leuserpin-2) is a single chain glycoprotein (Mr=65,600, 10% carbohydrate) member of the serine protease inhibitor (serpin) family (1-3). Amino acid sequence analysis of heparin cofactor II reveals 3 potential N-glycosylation sites and 2 sulfated tyrosines located in two internal repeats of 7 residues located near the NH2-terminal (4-6). The plasma concentration is estimated to be 90 µg/ml (2).

In the coagulation cascade, heparin cofactor II inhibits thrombin by formation of a bimolecular complex in the presence (k2=4.5x108 M-1min-1) and absence (k2=5.0x105 M-1min-1) of heparin (2, 7-9). This complex is stable to denaturants and essentially irreversible. In addition to heparin, a wide variety of polyanionic compounds accelerate inhibition of thrombin by HCII (3). Noteworthy among these compounds is dermatan sulfate, which accelerates inhibition of thrombin by HCII, but not antithrombin III (1,6) This feature has been utilized to develop a plasma based assay specific for heparin cofactor II. Although HCII shows only ~25% sequence homology to other serpins, the reactive site peptide (COOH-terminal 36 amino acids) released on inhibition of thrombin is 53% homologous to that released from antithrombin III (AT III) by thrombin (4,9). The scissile bond in HCII is Leu-Ser which is unusual for an arginine specific protease inhibitor (4,9). In contrast to AT III, the only coagulation protease inhibited by HCII is thrombin, Other proteases inhibited by HCII include α-chymotrypsin, neutrophil cathepsin G and streptomyces griseus protease B (3). The major physiologic role of heparin cofactor II may remain to be discovered.

Heparin cofactor II is prepared from fresh frozen plasma by a modification of the procedure of Griffith, et al., (9). Purity is assessed on SDS PAGE and activity is determined based on thrombin inhibition in the presence of heparin. HCII is supplied in 50% (vol/vol) glycerol/H20 for storage at -20°C.

品牌介绍
Haematologic Technologies是一家通过ISO 9001:2015认证的公司,是一家主要制造商,专门从事旨在用于体外研究的高质量血浆蛋白的分离和表征的主要制造商。HT的重点是参与凝血级联反应的蛋白质,以及骨代谢的调节。HT产品系列包括150多种高度纯化且特性良好的蛋白质,包括酶原,酶,辅因子和抑制剂,以及单克隆和多克隆抗体的互补产品系列。还提供因子不足的血浆和定制的用于临床研究的定制采血管。提供的服务包括定制蛋白质纯化,蛋白质修饰,分析开发,合同制造和合同研究。